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Volume 51, Number 5, October 2018

Fungal immunomodulatory protein-fve could modulate airway remodel through by affect IL17 cytokine 


Yu-Tzu Lee, Chia-Ta Wu, Hai-Lun Sun, Jiunn-Liang Ko, Ko-Haung Lue


 

Background and purpose: 

Background
Asthma is one of the most common allergic diseases. Our previous studies have reported that FIP-fve in acute allergic mouse model can reduce inflammation, improve the balance of the Th1/Th2 system. However, the effects of reducing airway remodeling on FIP-fve is still unknown.
Objective
We hypothesized that orally administrated FIP-fve should be able to reduce airway remodeling in chronic allergic models.



 

Methods:

The chronic asthma animal model was established with 6–8 weeks female Balb/c mice. After intranasal challenges with OVA, the airway inflammation and AHR were determined by a BUXCO system. BALF was analyzed with Liu's stain and ELISA assay. Lung histopathologic changes and Collagen deposition were assayed with H&E, Masson's trichrome and IHC stain.



 

Results:

FIP-fve significantly decreased the number of infiltrating inflammatory cells and Th2 cytokines and increased Th1 cytokines in BALF and serum compared with the OVA sensitized mice. FIP-fve had a better effect than corticosteroid could reduce infiltrating cells in lung especially neutrophils and eosinophils. We also found that the oral FIP-fve group suppressed IL-17 and enhanced IL-22 in the serum and BALF. In addition, oral FIP-fve decreased MMP9 expression, collagen expression and airway remodeling in lung tissues.

 



 

Conclusion:

FIP-fve had anti-inflammatory effects on OVA-induced airway inflammation and an effect to inhibited Th17 cells to reduced airway remodeling and collagen expression. Moreover, FIP-fve might be a potential alternative therapy for allergic airway diseases. 



 

Key words:

Asthma, Airway remodeling, Collagen deposition, FIP-fve, IL-17, IL-22