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Volume 50, Number 5, October 2017

The importance of matrix-assisted laser desorption ionization–time of flight mass spectrometry for correct identification of Clostridium difficile isolated from chromID C. difficile chromogenic agar 

Jonathan H.K. Chen, Vincent C.C. Cheng, Oi-Ying Wong, Sally C.Y. Wong, Simon Y.C. So, Wing-Cheong Yam, Kwok-Yung Yuen


Corresponding author:

Kwok-Yung Yuen, Corresponding author. Carol Yu Centre for Infection, University of Hong Kong, University Pathology Building, Queen Mary Hospital, Pokfulam, Hong Kong Special Administrative Region, China. 


Background and purpose: 

The clinical workflow of using chromogenic agar and matrix-assisted laser desorption ionization time-of-fight mass spectrometry (MALDI-TOF MS) for Clostridium difficile identification was evaluated. The addition of MALDI-TOF MS identification after the chromID C. difficile chromogenic agar culture could significantly improve the diagnostic accuracy of C. difficile. 


Key words:

chromogenic agar, Clostridium difficile, matrix-assisted laser desorption ionization–time of flight