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Volume 50, Number 1, February 2017

Detection of Pseudomonas aeruginosa isolates carrying the blaOXA-142 extended-spectrum β-lactamase gene in Taiwan 


Chang-Pan Liu, Te-Li Chen, Nai-Yu Wang, Shan-Fan Chow, Jung-Chung Lin, Tsong-Rong Yan


 

Corresponding author:

Jung-Chung Lin, Corresponding author. Division of Infectious Diseases and Tropical Medicine, Department of Internal Medicine, Tri-Service General Hospital, No. 325, Sector 2, Chenggong Road, Neihu District, Taipei, Taiwan. 



 

Background and purpose: 

The emergence of extended-spectrum β-lactamase (ESBL) OXA-142 gene (blaOXA-142) in Pseudomonas aeruginosa has been reported in Bulgaria and other European countries, but rarely in Asia. 



 

Methods:

From January 2009 to December 2012, 90 P. aeruginosa isolates with reduced susceptibility or resistance to ceftazidime (minimum inhibitory concentration ≥ 8 mg/L) were screened for ESBL and other broad-spectrum β-lactamase genes by polymerase chain reaction and sequencing. Clonal relationship of the isolates was determined by pulsed-field gel electrophoresis. 



 

Results:

Three isolates were positive for ESBL production, exhibited resistance to ceftazidime, and carried the blaOXA-142 gene. The blaOXA-142 gene was integrated into class 1 integron. Using Southern blot analysis, blaOXA-142-containing integron was found to be located on a plasmid in all three isolates. Eleven strains of P. aeruginosa carrying blaOXA-17 gene were found. The oprD mutation was found in all the 21 ESBL strains of P. aeruginosa. 



 

Conclusion:

This study confirmed the presence of blaOXA-142-positive P. aeruginosa isolates in Taiwan. 



 

Key words:

Extended-spectrum β-lactamase, Integron, OXA-142 gene, Pseudomonas aeruginosa