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Volume 50, Number 1, February 2017

Microbial communities associated with anaerobic degradation of polybrominated diphenyl ethers in river sediment
 


Chu-Wen Yang, Huang-Wen Huang, Bea-Ven Chang


 

Corresponding author:

Bee-Ven Chang, Corresponding author. Department of Microbiology, Soochow University, 70 Linhsi Road, Shihlin, Taipei 111, Taiwan. 



 

Background and purpose: 

Polybrominated diphenyl ethers (PBDEs) are extensively used as a class of flame retardants and have become ubiquitous environmental pollutants. We aimed to uncover the changes in microbial community with PBDE anaerobic degradation with and without zero-valent iron in sediment from the Erren River, considered one of the most heavily contaminated rivers in Taiwan. 



 

Methods:

PBDE anaerobic degradation in sediment was analyzed by gas chromatography with an electron capture detector. Microbial community composition was analyzed by a pyrosequencing-based metagenomic approach.

 



 

Results:

The anaerobic degradation rate of BDE-209 was higher than BDE-28 in sediment; the addition of zero-valent iron enhanced the degradation rates of both. In total, 19 known bacterial genera (4 major genera: Clostridium, Lysinibacillus, Rummeliibacillus, and Brevundimonas) were considered PBDE degradation-associated bacteria (sequence frequency negatively correlated with PBDE remaining percentage) as were four known archaea genera (Methanobacterium, Methanosarcina, Methanocorpusculum, and Halalkalicoccus; sequence frequency positively correlated with PBDE remaining percentage). 



 

Conclusion:

The composition of bacteria and that of archaea affected the anaerobic degradation of BDE-28 and BDE-209. The addition of zero-valent iron further decreased the archaea content to undetectable levels. 



 

Key words:

anaerobic degradation, BDE-28, BDE-209, microbial community