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Volume 49, Number 3, June 2016

Isolation and quantitation of Clostridium difficile in aqueous and fecal matter using two types of selective media 


Seth T. Housman, Mary Anne Banevicius, Lucinda M. Lamb, David P. Nicolau


 

Corresponding author:

Corresponding author. David P. Nicolau, Center for Anti-Infective Research and Development, Hartford Hospital; 80 Seymour Street, Hartford, CT 06102, USA. 



 

Background and purpose: 

We evaluated the isolation and quantitation of Clostridium difficile from aqueous and fecal samples utilizing ChromID CDIF and cycloserine, cefoxitin, and fructose-containing agar with horse blood and taurocholate media. Growth was similar between the two. ChromID CDIF provided enhanced isolation and required no ethanol pretreatment to inhibit normal flora. ChromID CDIF also improved turn-around time, requiring only 24 hours' incubation. 



 

Key words:

Cycloserine, cefoxitin, and fructose-containing agar with horse blood and taurocholate, Chromogenic agar, Clostridium difficile