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Volume 49, Number 3, June 2016

Effect of the HBV whole-X gene on the expression of hepatocellular carcinoma associated proteins

Yu Zhang, Hongli Liu, Meiling Cui, Jinfeng Liu, Ruitian Yi, Yinghua Niu, Tianyan Chen, Yingren Zhao
Yingren Zhao

Received: January 15, 2014       Accepted: July 15, 2014   


Corresponding author:

Corresponding author. Yingren Zhao, Department of Infectious Diseases, First Affiliated Hospital of Medical College, Xi'an Jiaotong University, Xi'an 710061, Shaanxi Province, China. 


Background and purpose: 

The hepatitis B virus (HBV) pre-X gene resides upstream of the HBV X gene, and together they form the HBV whole-X gene. Although it has been evident that the HBV whole-X protein is involved in the development of hepatocellular carcinoma, its biological role and molecular mechanism remain largely unknown. 



In this study, we subcloned the HBV whole-X gene and constructed a HBV whole-X expressing vector. After transfection of the HBV whole-X gene into HL-7702 cells, the profile of the differential cellular protein composition in the cells was analyzed by using two-dimensional electrophoresis coupled to matrix-assisted laser desorption/ionization-time of flight mass spectrometry. 



The results showed that 18 major proteins were differentially expressed in the cells transfected with or without the HBV whole-X gene. The expression of these genes was further confirmed by reverse transcription-polymerase chain reaction and Western blot analysis. 



Our findings provide a new insight into the investigation of the pathological role that the HBV whole-X gene plays in the development of hepatocellular carcinoma and may lead to the design of novel strategies for the treatment of this disease. 


Key words:

HBV whole-X gene, Hepatitis B virus, hepatocellular carcinoma