Print E-mail
Volume 48, Number 6, December 2015

In vitro interactions between fluconazole and minocycline against mixed cultures of Candida albicans and Staphylococcus aureus 


Hui Li, Caiqing Zhang, Ping Liu, Weiguo Liu, Yuan Gao, Shujuan Sun


Received: August 29, 2013    Revised: October 9, 2013    Accepted: March 26, 2014   

 

Corresponding author:

Shujuan Sun
Corresponding author. Department of Pharmacy, Qianfoshan Hospital Affiliated to Shandong University, No. 16766 Jingshi Road, Jinan 250014, Shandong Province, China. 



 

Background and purpose: 

It is difficult to manage coinfections in critically ill patients, especially in the presence of mixed-species biofilms. The aim of this study was to seek an effective drug combination for managing the dual-species biofilm of Candida albicans and Staphylococcus aureus. 



 

Methods:

The interaction between fluconazole and minocycline against polymicrobial planktonic cells and polymicrobial biofilms formed over four different time intervals (4 hours, 8 hours, 12 hours, and 24 hours) was investigated using a microdilution checkerboard method. To explore whether the combined effects against the polymicrobial cultures involved calcium regulation, the effects of benidipine and ethylene glycol tetraacetic acid were characterized using a plate streaking method and a liquid-based quantitative method. 



 

Results:

Fluconazole combined with minocycline exerted strong effects against polymicrobial planktonic cells and polymicrobial biofilms formed over 4 hours, 8 hours, and 12 hours. The addition of benidipine and ethylene glycol tetraacetic acid enhanced the activity of the drug combination, suggesting that the combined effects may involve the perturbation of calcium homeostasis. 



 

Conclusion:

Fluconazole in combination with minocycline is a potential approach for counteracting C. albicans–S. aureus dual-species biofilms. 



 

Key words:

Antimicrobial resistance, Calcium regulation, Candida albicans, Drug combination, Polymicrobial biofilms