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Volume 48, Number 6, December 2015

Inhibition of verotoxin (VT) 2 absorption into systemic blood from intestine by repeated administration of bovine immune colostral antibody against VT2 in mice 


Seita Tetsurou, Takashi Kuribayashi, Masafumi Fukuyama, Seiji Yamaguchi, Shizuo Yamamoto


Received: January 24, 2014    Revised: May 2, 2014    Accepted: May 28, 2014   

 

Corresponding author:

Shizuo Yamamota
Corresponding author. Graduate School of Environmental and Health Science, Azabu University, 1-17-71 Fuchinobe, Chou-ku Sagamihara, Kanagawa 252-5201, Japan. 



 

Background and purpose: 

Whether absorption of verotoxin (VT) 2 from the intestine in mice is inhibited by administration bovine immune colostral antibody against VT2 was investigated. 



 

Methods:

Three-week-old mice were administered VT2 solution at 477.8 ng/mL or 955.6 ng/mL, and bovine immune colostral antibody against VT2 was then administered three times. Whey without antibody against VT2 was administered to control mice. Serum levels of VT2 were measured by fluorescence enzyme immunoassay. 



 

Results:

Serum levels of VT2 in mice administered VT2 solution at 477.8 ng/mL and bovine immune colostral antibody against VT2 scarcely changed. By contrast, serum levels of VT2 in control mice increased and peaked 12 hours after administration. Peak values were 15.4 ± 5.04 ng/mL. Furthermore, serum levels of VT2 at 12 hours and 16 hours in control mice were significantly higher than in mice administered bovine colostral antibody against VT2. Serum levels of VT2 in mice administered antibody at 955.6 ng/mL showed no significant differences between repeated administration of bovine immune colostral antibody and controls. 



 

Conclusion:

These results suggest that absorption of VT2 from the intestine was inhibited by repeated administration of bovine immune colostral antibody against VT2 at early stages of Escherichia coli O157:H7 infection, whereas VT2 in the intestine remained at low levels. 



 

Key words:

absorption, bovine colostral antibody, intestine, mice, VT2