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Volume 46, Number 4, August 2013

Induction of protective immunity against brucellosis in mice by vaccination with a combination of naloxone, alum, and heat-killed Brucella melitensis 16 M 

Yousef Motaharinia, Mohammad Ali Rezaee, Ahmad Rashidi, Ali Jalili, Mohammad Jafar Rezaie, Reza Shapouri, Werya Hossieni, Mohammad Reza Rahmani

Received: February 2, 2012    Revised: February 29, 2012    Accepted: March 16, 2012   


Corresponding author:

Department of Immunology, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran.
E-mail address: (M.R. Rahmani). 


Background and purpose: 

A T-helper cell type 1-specific response leads to the elimination of intracellular infection with Brucella. Studies have shown that naloxone (NLX) can promote a cellular immune response in this respect. The current study was carried out to evaluate the induction
of protective immunity in mice against brucellosis by vaccination with a combination of NLX, alum, and heat-killed Brucella melitensis 16 M (HKB) 



Mice were categorized into five groups and received intraperitoneal vaccination on Days 0 and 7. Then serum levels of interferon (IFN)-g and interleukin (IL)-4, the bacterial load, and the survival rate were measured 2 weeks after the last vaccination. 



The serum levels of IFN-g, IL-4, and immunoglobulin G in the NLX þ alum þ HKB group were shown to be significantly increased (p < 0.05). Furthermore, the lowest bacterial load was observed in this group. The survival rate in groups vaccinated with combinations containing adjuvants was 100%. 



The combination of NLX and alum enhanced the immunogenicity of HKB, which can be used in the vaccination of animals and humans at risk of the disease. 


Key words:

Alum; Brucella melitensis 16 M;Naloxone; Protective immunity