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Volume 46, Number 2, April 2013

Comparison of efficacies of bovine immune colostral antibody and each immunoglobulin class against verotoxin 2, flagellum and somatic cells of Escherichia coli O157:H7 in mice 


Tetsurou Seita, Takashi Kuribayashi, Toshio Honjo, Shizuo Yamamoto
Corresponding author. Shizuo Yamamoto, Laboratory of Immunology, School of Life and Environmental Science, Azabu University, Fuchinobe 1-17-71, Sgamihara, Chuo-ku, Kanagawa 252-5201, Japan. E-mail address: yamamoto@azabu-u.ac.jp (S. Yamamoto)

Received: November 23, 2011    Revised: December 16, 2011    Accepted: December 30, 2011   

 

Corresponding author:

 * Corresponding author. Shizuo Yamamoto. Laboratory of Immunology, School of Life and Environmental Science, Azabu University, Fuchinobe 1-17-71,
Sgamihara, Chuo-ku, Kanagawa 252-5201, Japan.
E-mail address: yamamoto@azabu-u.ac.jp (S. Yamamoto).



 

Background and purpose: 

The efficacy of bovine immune colostral (colostral) antibodies against verotoxin (VT) 2, flagellum and somatic cells of Escherichia coli (E. coli) O157:H7 in mice was determined.



 

Methods:

Three major immunoglobulin (Ig) classes were isolated from the colostral antibody against VT2 by affinity chromatography and were used for estimation. Mice inoculated with VT2 were administered each Ig class from the colostral antibody, colostral antibody (colostral whey containing antibody) or serum antibody against VT2 at 1 hour after VT2 inoculation. 



 

Results:

All control mice (20/20) died after administration of sterilized saline instead of the colostral antibody. The survival rate was 93.3% (14/15) after administration of S-IgA or IgM antibody, or colostral antibody. Survival rates for IgG antibody and serum antibody administration were 80% (12/15) and 60% (9/15), respectively. Serum concentrations of VT2, which was absorbed from the small intestine in mice after administration of VT2 and colostral antibody,
were measured by fluorescence enzyme immunoassay (FEIA). Serum concentrations of VT2 after administration of colostral antibody were lower than those after administration of sterilized
saline. Mice inoculated with VT2-producing E. coli 157:H7 were administered antiflagellum or anti-somatic colostral antibodies. Survival rates for E. coli O157:H7-infected mice administered the anti-flagellum and anti-somatic colostral antibodies were 52.4% (11/21) and 22.2% (4/18), respectively. Furthermore, survival rates increased to 89.5% (17/19) with combined administration of anti-flagellum and anti-VT2 colostral antibodies. 



 

Conclusion:

These results suggest that colostral antibodies against VT2, flagellum and somatic cells are effective against E. coli O157:H7 infection. 



 

Key words:

E coli O157:H7; Flagellum mice; Immunoglobulin class; VT2