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Volume 45, Number 3, June 2012

Molecular detection and incidence of human papillomavirus in neonates: Methodology and a pilot study in a medical center


Chun-Fu Tai, Tsung-Pei Tsou, Wu-Shiun Hsieh, Chien-Yi Chen, Hung-Chieh Chou, Po-Nien Tsao, Chien-hui Hsu, Yi-Jen Liau, Chun-Yi Lu, Pei-Lan Shao, Luan-Yin Chang, Li-Min Huang*


Received: April 29, 2011    Revised: July 21, 2011    Accepted: July 31, 2011   

 

Corresponding author:

Department of Pediatrics, National Taiwan University Hospital, College of Medicine, Number 8, Chung-Shan South Road, Taipei 100, Taiwan. E-mail address: lmhuang@ntu.edu.tw (L.-M. Huang).



 

Background and purpose: 

 Human papillomavirus (HPV) infection can cause laryngeal papillomas in children. Vertical transmission has been confirmed. This study aimed to establish a sensitive molecular diagnostic method and understand the incidence of the HPV-6 and HPV-11 in neonates with intubation.



 

Methods:

We enrolled 108 newborns between October 2007 and January 2010. All neonates were intubated due to underlying disease. The specimens were collected via endotracheal aspiration. DNA of HPV types 6 and 11 was detected by real-time PCR and nested PCR.

 
tested positive for HPV-11 and one was positive for HPV-6. The HPV 6/11 detection rate
in neonates was 7.4% (8/108)


 

Results:

HPV-DNA was detected in eight of the 108 newborns studied. Seven respiratory specimens tested positive for HPV-11 and one was positive for HPV-6. The HPV 6/11 detection rate in neonates was 7.4% (8/108).



 

Conclusion:

A rapid, sensitive, specific, and reproducible RT-PCR method and nest PCR were developed for the detection and differentiation of HPV-6 and HPV-11 genomic variants in a single PCR reaction. The assays are of great value for clinical and epidemiologic studies of HPV-6 and HPV-11 infections. Neonatal HPV colonization may be related to juvenile-onset

recurrent respiratory papillomatosis. The transmission route may be from mother to child.
The clinical significance of neonatal carriage of HPV-6 or HPV-11 warrants further study.


 

Key words:

Colonization; HPV; Human papillomavirus; Neonate; Polymerase chain reaction