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Volume 44, Number 1, February 2011

Isolation of Campylobacter sp in surface waters of Taiwan


Tai-Lee Hu, Pao-chi Kuo


Received: April 8, 2009    Revised: November 14, 2009    Accepted: December 13, 2009   

 

Corresponding author:

Tai-Lee Hu

Department of Environmental Engineering and Science, Feng Chia University, #100 Wen-Hwa Road,
Taichung, 40724, Taiwan.
E-mail address: tlhu@fcu.edu.tw (T.-L. Hu).



 

Background and purpose: 

Campylobacter sp is one of the main causes of human acute enteritis and diarrhea. It is commonly found in bird intestinal canal and pollutes water bodies through bird droppings. Clinically, detection of Campylobacter sp is by culture method and most are fecal samples. The objective of this study is to investigate the distribution of Campylobacter sp in river water of Taiwan.



 

Methods:

The detection of campylobacters in water by the United Kingdom Health Protection Agency was adopted and simplified. Water samples (1 L) were enriched through membrane filters and selective enrichment Bolton broth. The mCCDA medium was used for culturing Campylobacter sp. In addition to using the selective media, DNA sequencing for species’ identification was also included in this study.



 

Results:

Campylobacter sp was detected in only 2 of 75 water samples, and 72 suspected strains were isolated from the selective mCCDA medium, in which several pathogenic bacteria included Escherichia coli O157.



 

Conclusion:

Not only there was pollution of Campylobacter sp in water environment but also other antibiotics-resistant pathogenic bacteria, which may cause public health risk.



 

Key words:

pathogens; Campylobacter sp; Selective enrichment medium