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Volume 44, Number 1, February 2011

Human colostral phagocytes eliminate enterotoxigenic Escherichia coli opsonized by colostrum supernatant


Eduardo Luzı´a Franc¸ Renata Vieira Bitencourt, Mahmi Fujimori, Tassiane Cristina de Morais, Iracema de Mattos Paranhos Calderon, Adenilda Cristina Honorio-Franc¸a


Received: March 2, 2009    Revised: October 14, 2009    Accepted: December 17, 2009   

 

Corresponding author:

Adenilda Cristina Honorio-Franc¸a
Institute of Biological Sciences and Health, Federal of University of Mato Grosso, Rodovia MT 100, Km 3,5 s/no, Pontal do Araguaia, MT 78698-000, Brazil.
E-mail address: adenilda@ufmt.br (A.C. Honorio-Franc¸a)



 

Background and purpose: 

Several elements in colostrumand human milk, including antibodies and nonspecific factors with bactericidal and antiviral activity, may play an important anti-infectious and protective role. In developing countries, enterotoxigenic Escherichia coli (ETEC) is the main etiological agent of diarrhea in low-socioeconomic level children. In the present work, we studied the functional activity ofmononuclear (MN) and polymorphonuclear (PMN) phagocytes of human colostrum against ETEC, as well as the interactions between these cells and colostral or serum opsonins.



 

Methods:

Colostrumsamples were collected from 33 clinically healthy women between 48 and 72 hours postpartum. We verified superoxide release in colostral MN and PMN using cytochrome C reduction methods,  phagocytosis, and bactericidal activity using acridine orange methods and superoxide dismutase (SOD) in the colostrum supernatants.



 

Results:

Colostral MN and PMN phagocytes exposed to ETEC opsonized with colostrum supernatants caused a significant increase (p < 0.05) in superoxide release. Phagocytosis by colostral PMN cells increased significantly (p < 0.5) when the phagocytes were incubated with both sources of opsonins (sera and colostrum). Increases in superoxide release in the presence of opsonized bacteria triggered the bactericidal activity of the phagocytes. Phagocyte treatment with SOD decreased their ability to eliminate ETEC. Colostrum supernatant had higher SOD concentrations
(p< 0.05) compared with normal human sera.



 

Conclusion:

These results suggest that the ability of phagocytes to eliminate ETEC depends on the activation of cellular oxidative metabolism; moreover, activation of colostral phagocytes is likely an additional breast-feeding protection mechanism against intestinal infections in infants



 

Key words:

Colostrum; Enterotoxigenic Escherichia coli; Microbicidal activity; Phagocytes