Julia Pasligh, Clarissa Radecke, Michael Fleischhacker*, Markus Ruhnke Division of Oncology, Department of Medicine, Charit. Universit.tsmedizin Berlin, Charit. Campus Mitte, Berlin, Germany
Received: September 2, 2008 Revised: October 8, 2008 Accepted: November 7, 2008
Background and purpose:
Mixed infections caused by different Candida species are the rule rather than the exception. The discrimination between the two closely related species Candida albicans and Candida dubliniensis is not trivial. Therefore, there is a need for fast, reliable, and inexpensive methods with high specificity for the identification and differentiation of these two Candida species, which are frequently detected in the oral cavities of patients with a human immunodeficiency virus infection.
We applied several phenotypic identification methods (growth on Rice-agar, Bird-seed agar, CHROMagar(R) Candida, API ID 32C; growth at 42.C and 45.C) and compared them with genotyping by arbitrarily primed-polymerase chain reaction.
C. dubliniensis from C. albicans in routine practice.
API ID 32C, AP-PCR, Bird-seed agar, Candida dubliniensis, CHROMagar(R) Candida