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Volume 37, Number 4, August 2004

Cloning, sequencing and expression of GroEL-like protein gene of Vibrio parahaemolyticus


Hin-Chung Wong, Kai-Hsi Lu
Department of Microbiology, Soochow University, Taipei, Taiwan, ROC

Received: December 19, 2003    Revised: March 25, 2004    Accepted: April 12, 2004   

 

Corresponding author:

Dr. Hin-Chung Wong, Department of Microbiology, Soochow University, 70 Lin-Si Rd., Taipei, Taiwan 111, ROC. E-mail: wonghc@mail.scu.edu.tw This e-mail address is being protected from spam bots, you need JavaScript enabled to view it

 



 

Methods:

Vibrio parahaemolyticus is an important enteropathogen in regions where much seafood is consumed. Substantial quantity of GroEL-like protein is produced during the heat shock of V. parahaemolyticus and located in periplasmic and extracellular fractions. In this study, the GroEL-like protein gene of this pathogen was cloned and sequenced and its properties were analyzed. The open reading frame consisted of 1647 bp, encoded a 57.6-kDa GroEL-likae protein of 548 amino acids. The amino acid sequence, hydrophobicity and antigenic pattern of V. parahaemolyticus GroEL-like protein were similar to the GroEL proteins of other vibrios, Escherichia coli and Salmonella enterica Typhi. The groEL-like gene of V. parahaemolyticus was cloned into the pQE-30 expression vector and the expression of a His-tagged GroEL-like protein was rapidly induced by isopropyl-β-D-thiogalactopyranoside, largely in an insoluble form. The results of this study facilitate the study of the functions of the GroEL-like protein of V. parahaemolyticus.



 

Key words:

 Base sequence, GroEL protein, heat-shock proteins, Vibrio parahaemolyticus

 



 

J Microbiol Immunol Infect 2004;37:203-207.