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Volume 37, Number 4, August 2004

Cloning, sequencing and expression of GroEL-like protein gene of Vibrio parahaemolyticus

Hin-Chung Wong, Kai-Hsi Lu
Department of Microbiology, Soochow University, Taipei, Taiwan, ROC

Received: December 19, 2003    Revised: March 25, 2004    Accepted: April 12, 2004   


Corresponding author:

Dr. Hin-Chung Wong, Department of Microbiology, Soochow University, 70 Lin-Si Rd., Taipei, Taiwan 111, ROC. E-mail: This e-mail address is being protected from spam bots, you need JavaScript enabled to view it




Vibrio parahaemolyticus is an important enteropathogen in regions where much seafood is consumed. Substantial quantity of GroEL-like protein is produced during the heat shock of V. parahaemolyticus and located in periplasmic and extracellular fractions. In this study, the GroEL-like protein gene of this pathogen was cloned and sequenced and its properties were analyzed. The open reading frame consisted of 1647 bp, encoded a 57.6-kDa GroEL-likae protein of 548 amino acids. The amino acid sequence, hydrophobicity and antigenic pattern of V. parahaemolyticus GroEL-like protein were similar to the GroEL proteins of other vibrios, Escherichia coli and Salmonella enterica Typhi. The groEL-like gene of V. parahaemolyticus was cloned into the pQE-30 expression vector and the expression of a His-tagged GroEL-like protein was rapidly induced by isopropyl-β-D-thiogalactopyranoside, largely in an insoluble form. The results of this study facilitate the study of the functions of the GroEL-like protein of V. parahaemolyticus.


Key words:

 Base sequence, GroEL protein, heat-shock proteins, Vibrio parahaemolyticus



J Microbiol Immunol Infect 2004;37:203-207.