Correlation of viral factors with cervical cancer in Taiwan
Yu-Yen Yang, Lim-Woh Koh, Ju-Hsin Tsai, Chung-Hung Tsai, Eric Fook-Chuen Wong, Shyh-Jye Lin, Chi-Chiang Yang
Departments of Medical Research, Clinical Laboratory and Obstetrics and Gynecology, Show Chwan Memorial Hospital, Changhua; Departments of Surgery and Pathology, Chung Shan Medical University Hospital, Taichung; and School of Medical Technology, Chung Shan Medical University, Taichung, Taiwan, ROC
Received: January 19, 2004 Revised: April 7, 2004 Accepted: May 7, 2004
The correlation of viral factors with cervical cancer was investigated. 27 cervical cancer biopsies and 29 normal cervical scrapings were determined by polymerase chain reaction method for 6 viruses, including human papillomavirus (HPV), cytomegalovirus (CMV), Epstein-Barr virus (EBV), herpes simplex virus (HSV)-1, HSV-2, and human herpes virus (HHV)-8. Among 27 biopsies of cervical cancer, HPV was identified in 18. Of these HPV-positive specimens, 9 cases of HPV type 16 were identified, 2 cases of HPV type 18 and 1 case of mixed infection with HPV types 16 and 18 were identified. Among the HPV types detected, type-16 is the most closely associated with cervical cancer and type-18 ranks second. Of the remaining 6 cases, 1 case of HPV-45, 1 case of mixed infection with HPV type 35, CMV and HSV-2, and 4 cases of unidentified HPV type were also found. EBV, HSV-1 and HHV-8 were not found in the cervical cancer samples and might have no or little relationship with cervical cancer. Among the 29 specimens in the normal female control group, no viral infection was detected. The correlation of HPV with cervical cancer was significantly different between frozen tissues and paraffin-embedded tissues. Other viruses such as HSV-2 and CMV are not predictive of cervical cancer. They might not be involved in the oncogenic processes directly but might enhance the possibility of oncogenesis or infect cancer tissues opportunistically.
Cervical neoplasms, human papillomavirus, polymerase chain reaction, risk factors, tumor virus infection
J Microbiol Immunol Infect 2004;37:282-287.