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Volume 38, Number 6, December 2005

Mutagenicity study of butachlor and its metabolites using Salmonella typhimurium


Kuei-Yao Hsu, Hwai-Jeng Lin, Jen-Kun Lin, Wein-Shung Kuo, Yueh-Hsing Ou
Department of Anesthesiology, Buddhist Tzu Chi General Hospital, Shin-Dien, Taipei; Division of Gastroenterology, Department of Medicine, Veterans General Hospital, Taipei; Institute of Biochemistry and Molecular Biology, College of Medicine, National Taiwan University, Taipei; Department of Anesthesia, Cheng Hsin Rehabilitation Medical Center, Taipei; and Faculty of Medical Technology, School of Medical Technology and Engineering, National Yang Ming University, Taipei, Taiwan

Received: November 26, 2004    Revised: June 30, 2005    Accepted: July 21, 2005   

 

Corresponding author:

Dr. Yueh-Hsing Ou, Faculty of Medical Technology, School of Medical Technology and Engineering, National Yang Ming University, Taipei, Taiwan. E-mail: yhou@ym.edu.tw This e-mail address is being protected from spam bots, you need JavaScript enabled to view it

 



 

Methods:

Butachlor is the most commonly used herbicide in Taiwan and many other countries. It has been reported to be an indirect mutagen and carcinogen in various in vitro assay systems. Previous investigation has also demonstrated that butachlor stimulates cell proliferation, transforms normal embryonic cells, and induces stomach tumors in Spraque-Dawley rats. However, the mechanism of butachlor carcinogenicity is still not clear. In order to clarify the toxicologic and carcinogenic properties of butachlor, we proposed a metabolic pathway, and synthesized the authentic metabolites by chemical methods. In addition, we tested the mutagenicity of butachlor and these metabolites on Salmonella typhimurium. The results indicate that butachlor might manifest its carcinogenicity via the mutagenicity of its metabolic products. Although the molecular mechanism of butachlor-induced cellular toxicity is still not clear, it is likely that the cellular transformation ability of butachlor is partly associated with its mutagenicity.

 



 

Key words:

Acetamides, acetanilides, butachlor, mutagenicity tests, Salmonella typhimurium


 



 

J Microbiol Immunol Infect 2005;38:409-416.