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Volume 38, Number 6, December 2005

Effect of static electric field treatment on multiple antibiotic-resistant pathogenic strains of Escherichia coli and Staphylococcus aureus


Roha Kasra Kermanshahi, Mohammad Reza Sailani
Department of Microbiology, Department of Biology, Faculty of Science, University of Esfahan, Esfahan, I.R. Iran

Received: April 18, 2005    Revised: July 15, 2005    Accepted: September 16, 2005   

 

Corresponding author:

M.R. Sailani, Department of Biology, Faculty of Science, Esfahan University, Hezarjerib St., 81746, Esfahan, I.R. Iran. E-mail: m.sailani@sci.ui.ac.ir This e-mail address is being protected from spam bots, you need JavaScript enabled to view it or msailani@yahoo.com This e-mail address is being protected from spam bots, you need JavaScript enabled to view it

 



 

Methods:

This study evaluated the effect of a (4.5 kV/cm, 50 Hz) static electric field (SEF) on pathogenic strains of Escherichia coli and Staphylococcus aureus with multiple antibiotic resistance. The bacteria were grown overnight at 37ºC in a nutrient broth medium, then inoculated in 5 mL fresh nutrient broth medium and incubated for 2 h at 25ºC with continuous shaking at 190 rpm. 10 × colony-forming units/mL of these bacteria were subjected to a 4.5 kV/cm, 50 Hz, SEF for various time periods. The effects of 5 different SEF exposure times (30, 60, 90, 120 and 150 min) on the bacteria were evaluated by the plate count agar method. The growth percentages of SEF treatment groups were significantly less than that of the control group. Inactivation significantly increased with the duration of SEF exposure. The results indicate that growth inhibition by SEF in the Gram-negative bacteria, E. coli, was greater than that in the Gram-positive bacteria, S. aureus. This study has demonstrated the antimicrobial effects of SEF treatment on 2 important pathogens, suggesting its potential for application as a method for controlling microbial population growth within in a variety of environments.

 



 

Key words:

Electromagnetic fields, Escherichia coli, multiple drug resistance, radiation effects, Staphylococcus aureus


 



 

J Microbiol Immunol Infect 2005;38:394-398.