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Volume 39, Number 3, June 2006

Comparison of immunoblot analysis of emergent multidrug-resistant Salmonella typhimurium definitive phage type 104 with a non-multidrug-resistant definitive phage type 104 strain


Yakubu B. Ngwai, Yoshikazu Adachi, Yasuki Ogawa, Kolo Ibrahim
Department of Microbiology, Human Virology and Biotechnology, National Institute for Pharmaceutical Research and Development, Abuja, Nigeria; and Animal Health Laboratory, School of Agriculture, Ibaraki University, Ibaraki, Japan

Received: August 15, 2005    Revised: August 31, 2000      

 

Corresponding author:

Dr. Yakubu Boyi Ngwai, Department of Microbiology, Human Virology and Biotechnology, National Institute for Pharmaceutical Research and Development, P.M.B. 21 Garki, Abuja, Nigeria. E-mail: ybngwai@yahoo.com This e-mail address is being protected from spam bots, you need JavaScript enabled to view it

 



 

Background and purpose: 

The emergent multidrug-resistant (MDR) Salmonella typhimurium definitive phage type 104 (DT104) is a public and veterinary health problem not only due to its wide host range and potential for enhanced virulence, but also the difficulty associated with its control. There is thus a need to investigate possible antigens of MDR DT104.

 



 

Methods:

Using standard protocols, whole cell lysates, outer membrane extracts and cell-free ultracentrifuge supernatants of selected isolates of MDR DT104 were prepared, electrophoretically separated and tested for their antigen-antibody reactivity in comparison with a non-MDR DT104 strain.

 



 

Results:

Protein antigens of both strain types were recognized by antibodies in chick serum in a similar manner for all methods of antigen preparation used.
 



 

Conclusion:

This study did not find differences between the antibody recognition of MDR DT104 and that of the non-MDR DT104 strain tested. This observation should strengthen the quest for the possible use of vaccines to control this emergent strain in poultry.

 



 

Key words:

Antibody response, antigens, bacterial drug resistance, immunoblotting, proteome, Salmonella typhimurium DT104



 



 

J Microbiol Immunol Infect 2006;39:189-194.