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Volume 39, Number 5, October 2006

Characterization of various strains of influenza B virus in a neuroblastoma and a glioblastoma cell line

Chi-Ho Chan, Yuan-Ti Lee, Horng-Rong Chang, You Chan, Wu-Tse Liu
Department of Microbiology and Immunology, Chung Shan Medical University Taichung; Center for Clinical and Virus Research, Department of Clinical Laboratory and Divisions of Infectious Diseases and Nephrology, Department of Internal Medicine, Institute of Medicine, Chung Shan Medical University Hospital, Taichung; and The Examination Yuan, Taipei, Taiwan

Received: August 8, 2005    Revised: January 16, 2006    Accepted: January 18, 2006   


Corresponding author:

Dr. Chi-Ho Chan, 110, Sec. No. 1, Chien-Kuo N. Road, Taichung 402, Taiwan. E-mail: This e-mail address is being protected from spam bots, you need JavaScript enabled to view it



Background and purpose: 

Although influenza B virus has been reported to be involved in central nervous system infection, little is known about the infectivity of the virus. We evaluated the ability of several strains of influenza B virus to grow in 2 nerve cell culture systems.



Five isolates of influenza B virus were infected into a neuroblastoma cell line, IMR-32 and a human glioblastoma cell line, GBM 8401, respectively. To determine the permissiveness of these virus strains in both cells, the severity of the cytopathic effect (CPE), relative quantitative analysis with hemadsorption and hemagglutination, reverse transcriptase-polymerase chain reaction (RT-PCR) as well as kinetic study of viral protein synthesis were performed.




All tested viruses grew well in IMR-32, but only B/3143/Taiwan/97 replicated well in GBM 8401, according to the results of CPE, hemagglutination, hemadsorption, RT-PCR and viral protein synthesis.




Besides the binding of viral receptor and hemagglutinin being critical for a permissive infection, the interaction of other virus proteins and the other unknown host factors might also affect the ability of influenza B virus to infect a host cell.



Key words:

Influenza B virus, tumor cell line, viral growth, virulence



J Microbiol Immunol Infect2006;39:380-386.