Print E-mail
Volume 39, Number 6, December 2006

Real-time polymerase chain reaction assay for detection of Streptococcus pneumoniae in sputum samples from patients with community-acquired pneumonia


Aysen Bayram, Esra Kocoglu, Iclal Balci, Ayten Filiz, Fahriye Eksi
Departments of Microbiology and Clinical Microbiology and Pulmonary Diseases, Gaziantep University Medical Faculty, Gaziantep, Turkey

Received: November 1, 2005    Revised: February 20, 2006    Accepted: March 9, 2006   

 

Corresponding author:

Dr. Aysen Bayram, Gaziantep University Medical Faculty, Department of Microbiology and Clinical Microbiology, Universite Bulvari, Gaziantep 27310, Turkey. E-mail: aysenbayram@hotmail.com This e-mail address is being protected from spam bots, you need JavaScript enabled to view it

 



 

Background and purpose: 

A quantitative real-time polymerase chain reaction (PCR) method targeting the pneumolysin gene of Streptococcus pneumoniae in sputum specimens from patients with community-acquired pneumonia (CAP) was evaluated for the identification of pneumococci.
 



 

Methods:

The applicability of the assay to clinical samples was evaluated by studying 140 sputum specimens from patients with CAP. Of the specimens, 96 (68.6%) were found to be positive by real-time PCR. The results were compared to culture findings.

 



 

Results:

The sensitivity and specificity of the real-time PCR assay developed in this study as compared to those of the culture method were 97.2% and 60.9%, respectively.

 



 

Conclusion:

Real-time PCR assay was found to be a rapid and sensitive method for the detection of pneumococci.

 



 

Key words:

Pneumonia, polymerase chain reaction, sensitivity and specificity, sputum, Streptococcus pneumoniae


 



 

J Microbiol Immunol Infect 2006;39:452-457.