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Volume 33, Number 2, June 2000

Application of representational difference analysis to cloning a Mycoplasma arthritidis specific DNA fragment


Chiung-Sui Kuo, Hong-Shang Hong, Jwo-Farn Chiou
Graduate Institute of Microbiology, College of Medicine, National Taiwan University, Taipei, ROC

 

Methods:


Representation difference analysis (RDA) was applied to isolate a Mycoplasma arthritidis specific DNA fragment. The DNA fragment obtained was verified to be M. arthritidis specific by polymerase chain reaction (PCR) and dot blot hybridization tests. The size of this fragment was 194 bp and the nucleotide sequence was also determined.



 

J Microbiol Immunol Infect 2000;33:127-130.