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Volume 33, Number 2, June 2000

Preparation of Der p 1 specific monoclonal antibodies and use in a two-site-ELISA to detect Der p 1 allergen


Tzu-I Chien, Yi-Guang Chen, Bor-Luen Chiang
Graduate Institute of Immunology, College of Medicine, National Taiwan University, Taipei, ROC

 

Methods:

It has been well documented that the mite Dermatophoides pteronyssinus (Der p) is the major allergen in house dust in Taiwan. The purpose of this study is to develop a sandwich immunometric assay which can be used for the standardization of mite allergen extracts or house dust, for assessing the procedures of mite avoidance, and for identifying species in epidemiological studies. Der p 1 allergen (both recombinant and native Der p 1) was purified and a panel of Der p 1 specific monoclonal antibodies was developed. The monoclonal antibodies were biotinylated and a two-site enzyme-linked immunosorbent assay (ELISA) with peroxidase-conjugated streptavidin was developed for the detection of Der p 1 allergen. The results suggest that these monoclonal antibodies could be applied both in affinity columns for the purification of native Der p 1 antigen and in sandwich-ELISA for epidemiological study.



 

J Microbiol Immunol Infect 2000;33:87-92.