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Volume 41, Number 2, April 2008

Isolation of urease-positive Ochrobactrum intermedium in the stomach of a non-ulcer dyspeptic patient from north India


Mahesh S. Dharne, Sri Prakash Misra, Vatsala Misra, Manisha Dwivedi, Milind S. Patole, Yogesh S. Shouche
Molecular Biology Unit, National Centre for Cell Science, Pune; and Departments of Gastroenterology and Pathology, Moti Lal Nehru Medical College, Allahabad, India

Received: October 19, 2006    Revised: February 1, 2007    Accepted: March 27, 2007   

 

Corresponding author:

Dr. Yogesh S. Shouche, Molecular Biology Unit, National Centre for Cell Science, Ganeshkhind Road, Pune University Campus, Pune 411 007, Maharashtra, India. E-mail: Dr. Yogesh S. Shouche This e-mail address is being protected from spam bots, you need JavaScript enabled to view it

 



 

Methods:

Ochrobactrum intermedium is an opportunistic human pathogen found in immunocompromised individuals. We report the case of a north Indian patient with non-ulcer dyspepsia whose gastric biopsy revealed the presence of O. intermedium, along with Helicobacter pylori. Further description of O. intermedium was performed with 16S rRNA (1500 nucleotides) and RecA (1065 nucleotides) gene sequencing, and the identity and phylogenetic affiliation of the isolate was confirmed by 100% nucleotide similarity with O. intermedium LMG3301. Further investigation is required in order to evaluate the link between H. pylori and O. intermedium in the gastric niche.

 



 

Key words:

Helicobacter pylori; Ochrobactrum; RecA recombinases; RNA, ribosomal, 16S


 



 

J Microbiol Immunol Infect. 2008;41:183-186.