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Volume 41, Number 6, December 2008

Phenotypic and molecular identification of lactobacilli isolated from vaginal secretions


Silviya Petrova Dimitonova, Boris Vladimirov Bakalov, Ralitsa Nedkova Aleksandrova-Georgieva, Svetla Trifonova Danova


Received: March 27, 2007    Revised: September 20, 2007    Accepted: March 23, 2008   

 

Corresponding author:

Dr. Svetla Trifonova Danova, Department of Microbial Genetics, Institute of Microbiology, Bulgarian Academy of Sciences, 26 Acad. Georgi Bontchev St., 1113 Sofia, Bulgaria. E-mail: Dr. Svetla Trifonova Danova This e-mail address is being protected from spam bots, you need JavaScript enabled to view it
 



 

Background and purpose: 

Twenty lactobacilli isolated from vaginal samples of healthy volunteers were characterized according to polyphasic taxonomy. A broad spectrum of activity and protective properties of these vaginal isolates has been reported.

 



 

Methods:

Phenotypic and genotypic methods such as random amplified polymorphic DNA (RAPD), speciesspecific and BOX polymerase chain reaction (PCR), amplified ribosomal DNA restriction analysis (ARDRA), and 16S rRNA sequence analyses were applied for Lactobacillus spp. identification.
 



 

Results:

On the basis of carbohydrate utilization profiles using API 50 CHL kits (fermentation pattern), the strains were divided into 7 groups. RAPD- and BOX-PCR analyses revealed heterogeneity within the established phenotypic groups and discriminated successfully between the vaginal strains. The combination of species-specific PCR, ARDRA, and 16S rRNA sequence analyses allowed the species identification of 7 strains as Lactobacillus fermentum, 2 as Lactobacillus gasseri, 1 as Lactobacillus brevis, and 1 as Lactobacillus salivarius. These results were not in agreement with data obtained by classical and molecular methods for some clinical isolates.



 

Conclusion:

This is the first polyphasic taxonomy study on vaginal lactobacilli from Bulgarian women of reproductive age. These data add to the limited information existing in Bulgaria on the true identity of Lactobacillus spp. in the vaginal microbiota.

 



 

Key words:

Databases, genetic; DNA, ribosomal; Lactobacillus; Molecular sequence data; Random amplified polymorphic DNA technique; Vagina

 



 

J Microbiol Immunol Infect. 2008;41:469-477.