Upregulation of major histocompatibility complex class II, CD83, CD64, and CD14 on polymorphonuclear neutrophils stimulated with interleukin-15
Bahaa Kenawy Abuel-Hussien Abdel-Salam, Hossam Ebaid
Zoology Department, Faculty of Science, Minia University, Minia, Egypt
Received: August 24, 2006 Revised: July 1, 2007 Accepted: December 28, 2007
Background and purpose:
Polymorphonuclear neutrophils (PMNs) act as a first line of defense against bacterial infections. Recent studies indicated that PMNs could act as antigen-presenting cells. The aim of this study was to detect the expression of major histocompatibility complex (MHC) class II and certain molecules and receptors (CD83, CD64, and CD14) on the surface of PMNs stimulated with interleukin (IL)-15.
PMNs were isolated from heparinized whole blood of healthy volunteers. Cells were incubated for 48 h at 37°C in a humidified incubator with 5% carbon dioxide. After incubation for 48 h with and without IL-15, surface molecules were assayed by cytofluorometry.
Stimulation with IL-15 produced an increase in the expression of MHC class II and upregulation of the costimulatory molecules CD83, the immunoglobulin G receptor CD64, and the lipopolysaccharide receptor CD14 on the surface of PMNs
The expression of MHC class II, CD83, CD64, and CD14 indicates that PMNs may be involved in both innate and acquired immunity.
Antigens, CD; Interleukin-15; Major histocompatibility complex; Neutrophils; Receptors, IgG
J Microbiol Immunol Infect. 2008;41:462-468.