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Volume 41, Number 6, December 2008

Upregulation of major histocompatibility complex class II, CD83, CD64, and CD14 on polymorphonuclear neutrophils stimulated with interleukin-15


Bahaa Kenawy Abuel-Hussien Abdel-Salam, Hossam Ebaid
Zoology Department, Faculty of Science, Minia University, Minia, Egypt

Received: August 24, 2006    Revised: July 1, 2007    Accepted: December 28, 2007   

 

Corresponding author:

Dr. Bahaa Kenawy Abuel-Hussien Abdel-Salam, Zoology Department, Faculty of Science, Minia University, Minia, Egypt. E-mail: Dr. Bahaa Kenawy Abuel-Hussien Abdel-Salam This e-mail address is being protected from spam bots, you need JavaScript enabled to view it
 



 

Background and purpose: 

Polymorphonuclear neutrophils (PMNs) act as a first line of defense against bacterial infections. Recent studies indicated that PMNs could act as antigen-presenting cells. The aim of this study was to detect the expression of major histocompatibility complex (MHC) class II and certain molecules and receptors (CD83, CD64, and CD14) on the surface of PMNs stimulated with interleukin (IL)-15.
 



 

Methods:

PMNs were isolated from heparinized whole blood of healthy volunteers. Cells were incubated for 48 h at 37°C in a humidified incubator with 5% carbon dioxide. After incubation for 48 h with and without IL-15, surface molecules were assayed by cytofluorometry.
 



 

Results:

Stimulation with IL-15 produced an increase in the expression of MHC class II and upregulation of the costimulatory molecules CD83, the immunoglobulin G receptor CD64, and the lipopolysaccharide receptor CD14 on the surface of PMNs



 

Conclusion:

The expression of MHC class II, CD83, CD64, and CD14 indicates that PMNs may be involved in both innate and acquired immunity.
 



 

Key words:

Antigens, CD; Interleukin-15; Major histocompatibility complex; Neutrophils; Receptors, IgG

 



 

J Microbiol Immunol Infect. 2008;41:462-468.