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Volume 42, Number 2, April 2009

Induction of resistance to respiratory tract infection with Klebsiella pneumoniae in mice fed on a diet supplemented with tulsi (Ocimum sanctum) and clove (Syzgium aromaticum) oils

Archana Saini, Saroj Sharma, Sanjay Chhibber
Department of Microbiology, Panjab University, Chandigarh, India

Received: April 4, 2007    Revised: February 2, 2008    Accepted: May 21, 2008   


Corresponding author:

Dr. Sanjay Chhibber, Basic Medical Sciences Building, Department of Microbiology, Panjab University, Chandigarh 160014, India. E-mail: Sanjay Chhibber This e-mail address is being protected from spam bots, you need JavaScript enabled to view it


Background and purpose: 

The impact of diet and specific food groups on respiratory tract infections has been widely recognized in recent years. This study was conducted to study the effect of tulsi (Ocimum sanctum) oil and clove (Syzgium aromaticum) oil on the susceptibility of experimental mice to respiratory tract infection.



 The effect of 2 different regimens of short-term (15 days) and long-term (30 days) feeding with tulsi oil and clove oil on the course of Klebsiella pneumoniae American Type Culture Collection 43816 infection in the lungs of mice was analyzed. The operative mechanisms of lipid peroxidation/nitrite production were studied by estimating their levels in bronchoalveolar lavage fluid (BALF). Bacterial colonization, malondialdehyde (MDA) and nitrite production in BALF, and tumor necrosis factor–α level in serum were assessed.



The results showed that there was a significant decrease in bacterial colonization after short-term feeding with clove oil compared with the controls (p < 0.05). For tulsi oil–fed mice, the decrease in bacterial load was significant with long-term feeding (p < 0.01). The maximum decrease in MDA levels and increase in nitrite levels were noted with long-term feeding.



Dietary supplementation with tulsi and clove oils protects against bacterial colonization of the lungs.



Key words:

Clove oil; Klebsiella pneumoniae; Ocimum; Pneumonia


J Microbiol Immunol Infect. 2009;42:107-113.